Fluorescence Microscopy-Based Quantitation of GLUT4 Translocation: High Throughput or High Content?

ABSTRACT: Due to the global rise of type 2 diabetes mellitus (T2DM) in combination with insulin resistance, novel compounds to efficiently treat this pandemic disease are needed. Screening for compounds that induce the translocation of glucose transporter 4 (GLUT4) from the intracellular compartments to the plasma membrane in insulin-sensitive tissues is an innovative strategy. Here, we compared the applicability of three fluorescence microscopy-based assays optimized for the quantitation of GLUT4 translocation in simple cell systems. An objective-type scanning total internal reflection fluorescence (TIRF) microscopy approach was shown to have high sensitivity but only moderate throughput. Therefore, we implemented a prism-type TIR reader for the simultaneous analysis of large cell populations grown

SUBMITTER: Stadlbauer V 

PROVIDER: S-EPMC7662403 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature