Fatty acid nitroalkenes inhibit the inflammatory response to bleomycin-mediated lung injury.

ABSTRACT: Fatty acid nitroalkenes are reversibly-reactive electrophiles, endogenously detectable at nM concentrations, displaying anti-inflammatory actions. Nitroalkenes like 9- or 10-nitro-octadec-9-enoic acid (e.g. nitro-oleic acid, OA-NO₂) pleiotropically suppress cardiovascular inflammatory responses, with pulmonary responses less well defined. C57BL/6 J male mice were intratracheally administered bleomycin (3 U/kg, ITB), to induce pulmonary inflammation and acute injury, or saline and were treated with 50 μL OA-NO₂ (50 μg) or vehicle in the same instillation and 72 h post-exposure to assess anti-inflammatory properties. Bronchoalveolar lavage (BAL) and lung tissue were collected 7d later. ITB mice lost body weight, with OA-NO₂ mitigating this loss (-2.3 ± 0.94 v

SUBMITTER: Wilkinson ML 

PROVIDER: S-EPMC7662837 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature