Phospho-Mimetic Mutation at Ser602 Inactivates Human TRPA1 Channel.

ABSTRACT: The Transient Receptor Potential Ankyrin 1 (TRPA1) channel is an integrative molecular sensor for detecting environmental irritant compounds, endogenous proalgesic and inflammatory agents, pressure, and temperature. Different post-translational modifications participate in the discrimination of the essential functions of TRPA1 in its physiological environment, but the underlying structural bases are poorly understood. Here, we explored the role of the cytosolic N-terminal residue Ser602 located near a functionally important allosteric coupling domain as a potential target of phosphorylation. The phosphomimetic mutation S602D completely abrogated channel activation, whereas the phosphonull mutations S602G and S602N produced a fully functional channel. Using mutagenesis, electrophysiology, a

SUBMITTER: Barvikova K 

PROVIDER: S-EPMC7663402 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature