In Vivo Pre-Instructed HSCs Robustly Execute Asymmetric Cell Divisions In Vitro.

ABSTRACT: Hematopoietic stem cells (HSCs) are responsible for life-long production of all mature blood cells. Under homeostasis, HSCs in their native bone marrow niches are believed to undergo asymmetric cell divisions (ACDs), with one daughter cell maintaining HSC identity and the other committing to differentiate into various mature blood cell types. Due to the lack of key niche signals, in vitro HSCs differentiate rapidly, making it challenging to capture and study ACD. To overcome this bottleneck, in this study, we used interferon alpha (IFNα) treatment to "pre-instruct" HSC fate directly in their native niche, and then systematically studied the fate of dividing HSCs in vitro at the single cell level via time-lapse analysis, as well as multigene and protein expression analysis. Triggering HSCs'

SUBMITTER: Girotra M 

PROVIDER: S-EPMC7663432 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature