Recent Advances in Single-Particle Electron Microscopic Analysis of Autophagy Degradation Machinery.

ABSTRACT: Macroautophagy (also known as autophagy) is a major pathway for selective degradation of misfolded/aggregated proteins and damaged organelles and non-selective degradation of cytoplasmic constituents for the generation of power during nutrient deprivation. The multi-step degradation process, from sequestering cytoplasmic cargo into the double-membrane vesicle termed autophagosome to the delivery of the autophagosome to the lysosome or lytic vacuole for breakdown, is mediated by the core autophagy machinery composed of multiple Atg proteins, as well as the divergent sequence family of selective autophagy receptors. Single-particle electron microscopy (EM) is a molecular imaging approach that has become an increasingly important tool in the structural characterization of proteins and macromo

SUBMITTER: Cheung YWS 

PROVIDER: S-EPMC7663694 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature