Single-Cell RNA-Seq Reveals that CD9 Is a Negative Marker of Glucose-Responsive Pancreatic β-like Cells Derived from Human Pluripotent Stem Cells.

ABSTRACT: To date, it remains unclear if there are specific cell-surface markers for purifying glucose-responsive pancreatic β-like cells derived from human pluripotent stem cells (hPSCs). In searching for this, we generated an efficient protocol for differentiating β-like cells from human embryonic stem cells. We performed single-cell RNA sequencing and found that CD9 is a negative cell-surface marker of β-like cells, as most INS⁺ cells are CD9^-. We purified β-like cells for spontaneous formation of islet-like organoids against CD9, and found significantly more NKX6.1⁺MAFA⁺C-PEPTIDE⁺ β-like cells in the CD9^- than in the CD9⁺ population. CD9^- cells also demonstrate better glucose responsiveness than CD9⁺

SUBMITTER: Li X 

PROVIDER: S-EPMC7663789 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature