Targeting HMGB3/hTERT axis for radioresistance in cervical cancer.

ABSTRACT:

Background

Radiotherapy is regarded as a milestone for the cure of cervical cancer. However, clinical outcome heavily be hindered by radioresistance. So, exploring the underlying mechanism of radioresistance, and find potential target, well deserve fully emphasis.

Methods

In this study, we developed two novel radiation resistance cervical cancer cell lines, which could mimic clinical radioresistance. In order to find new potential targets, RNA-Seq, database analysis, streptavidin-agarose and LC/MS were used. Pull-down, luciferase and rescue assays were conducted to explore the regulatory mechanisms. To further evaluate the correlation between therapeutic responses and HMGB3/hTERT expression, 172 cervical cancer patients were recruited.

Results

Knockdown of HMGB3 signi

SUBMITTER: Li Z 

PROVIDER: S-EPMC7664109 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature