CRIPTO antagonist ALK4^L75A-Fc inhibits breast cancer cell plasticity and adaptation to stress.

ABSTRACT:

Background

CRIPTO is a multi-functional signaling protein that promotes stemness and oncogenesis. We previously developed a CRIPTO antagonist, ALK4^L75A-Fc, and showed that it causes loss of the stem cell phenotype in normal mammary epithelia suggesting it may similarly inhibit CRIPTO-dependent plasticity in breast cancer cells.

Methods

We focused on two triple negative breast cancer cell lines (MDA-MB-231 and MDA-MB-468) to measure the effects of ALK4^L75A-Fc on cancer cell behavior under nutrient deprivation and endoplasmic reticulum stress. We characterized the proliferation and migration of these cells in vitro using time-lapse microscopy and characterized stress-dependent changes in the levels and distribution of CRIPTO signaling mediators and cancer

SUBMITTER: Balcioglu O 

PROVIDER: S-EPMC7664111 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature