Localization of CDR2L and CDR2 in paraneoplastic cerebellar degeneration.

ABSTRACT:

Objective

Identify the subcellular location and potential binding partners of two cerebellar degeneration-related proteins, CDR2L and CDR2, associated with anti-Yo-mediated paraneoplastic cerebellar degeneration.

Methods

Cancer cells, rat Purkinje neuron cultures, and human cerebellar sections were exposed to cerebrospinal fluid and serum from patients with paraneoplastic cerebellar degeneration with Yo antibodies and with several antibodies against CDR2L and CDR2. We used mass spectrometry-based proteomics, super-resolution microscopy, proximity ligation assay, and co-immunoprecipitation to verify the antibodies and to identify potential binding partners.

Results

We confirmed the CDR2L specificity of Yo antibodies by mass spectrometry-based proteomics and found that

SUBMITTER: Herdlevaer I 

PROVIDER: S-EPMC7664253 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature