Investigating the Use of Impedance Flow Cytometry for Classifying the Viability State of E. coli.

ABSTRACT: Bacteria detection, counting and analysis is of great importance in several fields. When viability plays a major role in decision making, the counting of colony-forming units grown on agar plates remains the gold standard. However, because plate counts depend on the growth of the bacteria, it is a slow procedure and only works with culturable species. Impedance flow cytometry (IFC) is a promising technology for particle detection, counting and characterization. It relies on the perturbation of an electric field by particles flowing through a microfluidic channel. The perturbation is directly related to the electrical properties of the particles, and therefore provides information about their composition and structure. In this work we investigate whether IFC can be used to differentiate via

SUBMITTER: Bertelsen CV 

PROVIDER: S-EPMC7664255 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature