Long-term peripheral immune cell profiling reveals further targets of oral cladribine in MS.

ABSTRACT:

Objectives

To expand the knowledge about the immunological consequences of cladribine (CLAD), a pulsed immune reconstitution therapy approved for active multiple sclerosis (MS), beyond the known short-term effects on peripheral immune cell subsets.

Methods

In this study, we characterized depletion and restitution kinetics as well as cytokine profiles of peripheral immune cell subsets in 18 patients with MS following treatment with oral CLAD. The methods involved blood collection prior to CLAD and every three months over a period of 24 months, and extensive characterization of various immune cells subsets by multiparametric flow cytometry.

Results

We found a selectivity of CLAD towards central memory T cells and memory B cells and detected a hyper-repopulation of matur

SUBMITTER: Moser T 

PROVIDER: S-EPMC7664268 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature