Insights into a Protein-Nanoparticle System by Paramagnetic Perturbation NMR Spectroscopy.

ABSTRACT:

Background

The interaction between proteins and nanoparticles is a very relevant subject because of the potential applications in medicine and material science in general. Further interest derives from the amyloidogenic character of the considered protein, β2-microglobulin (β2m), which may be regarded as a paradigmatic system for possible therapeutic strategies. Previous evidence showed in fact that gold nanoparticles (AuNPs) are able to inhibit β2m fibril formation in vitro.

Methods

NMR (Nuclear Magnetic Resonance) and ESR (Electron Spin Resonance) spectroscopy are employed to characterize the paramagnetic perturbation of the extrinsic nitroxide probe Tempol on β2m in the absence and presence of AuNPs to determine the surface accessibility properties and the occurrence of c

SUBMITTER: Hunashal Y 

PROVIDER: S-EPMC7664681 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature