Selection of sponge-associated bacteria with high potential for the production of antibacterial compounds.

ABSTRACT: The potential of sponge-associated bacteria for the biosynthesis of natural products with antibacterial activity was evaluated. In a preliminary screening 108 of 835 axenic isolates showed antibacterial activity. Active isolates were identified by 16S rRNA gene sequencing and selection of the most promising strains was done in a championship like approach, which can be done in every lab and field station without expensive equipment. In a competition assay, strains that inhibited most of the other strains were selected. In a second round, the strongest competitors from each host sponge competed against each other. To rule out that the best competitors selected in that way represent similar strains with the same metabolic profile, BOX PCR experiments were performed, and extracts of these str

SUBMITTER: Riyanti 

PROVIDER: S-EPMC7665026 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature