Characterization of Gut Microbiome in Liver Transplant Recipients With Nonalcoholic Steatohepatitis.

ABSTRACT: Nonalcoholic fatty liver disease (NAFLD) and its progressive form nonalcoholic steatohepatitis (NASH) are a growing problem globally and recur even after liver transplant (LT). We aim to characterize the gut dysbiosis in patients who developed recurrent NAFLD compared with patients without recurrence following LT.

Methods

Twenty-one patients who received LT for NASH and had a protocol liver biopsy performed beyond 1-y post-LT were included prospectively (January 2018-December 2018). Genomic DNA extraction, next-generation sequencing, and quantitative PCR analysis were performed on stool samples collected within 1.1 ± 1.6 y from time of liver biopsy.

Results

Recurrent NAFLD was noted in 15 of the 21 included patients. Stool microbiome analysis at the genus level showed signifi

SUBMITTER: Satapathy SK 

PROVIDER: S-EPMC7665248 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature