MicroRNA alterations driving acute and late stages of radiation-induced fibrosis in a murine skin model.

ABSTRACT:

Purpose

Although ionizing radiation is critical in treating cancer, radiation-induced fibrosis (RIF) can have a devastating impact on patients' quality of life. The molecular changes leading to radiation-induced fibrosis must be elucidated so that novel treatments can be designed.

Methods and materials

To determine whether microRNAs (miRs) could be responsible for RIF, the fibrotic process was induced in the right hind legs of 9-week old CH3 mice by a single-fraction dose of irradiation to 35 Gy, and the left leg served as an unirradiated control. Fibrosis was quantified by measurements of leg length compared with control leg length. By 120 days after irradiation, the irradiated legs were 20% (P=.013) shorter on average than were the control legs.

Results

Tissue analy

SUBMITTER: Simone BA 

PROVIDER: S-EPMC7665809 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature