Unexpected photosensitivity of the well-characterized heme enzyme chlorite dismutase.

ABSTRACT: Chlorite dismutase is a heme enzyme that catalyzes the conversion of the toxic compound ClO₂^- (chlorite) to innocuous Cl^- and O₂. The reaction is a very rare case of enzymatic O-O bond formation, which has sparked the interest to elucidate the reaction mechanism using pre-steady-state kinetics. During stopped-flow experiments, spectroscopic and structural changes of the enzyme were observed in the absence of a substrate in the time range from milliseconds to minutes. These effects are a consequence of illumination with UV-visible light during the stopped-flow experiment. The changes in the UV-visible spectrum in the initial 200 s of the reaction indicate a possible involvement of a ferric superoxide/ferrous oxo or ferric hydroxide intermediate du

SUBMITTER: Mahor D 

PROVIDER: S-EPMC7665973 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature