Chromosome clustering by Ki-67 excludes cytoplasm during nuclear assembly.

ABSTRACT: Gene expression in eukaryotes requires the effective separation of nuclear transcription and RNA processing from cytosolic translation¹. This separation is achieved by the nuclear envelope, which controls the exchange of macromolecules through nuclear pores². During mitosis, however, the nuclear envelope in animal and plant cells disassembles, allowing cytoplasmic and nuclear components to intermix³. When the nuclear envelope is reformed, cytoplasmic components are removed from the nucleus by receptor-mediated transport through nuclear pores². These pores have a size limit of 39 nanometres^4-7, which raises the question of how larger cytoplasmic molecules are cleared from the nucleus. Here we show in HeLa cells that large cytoplasmic co

SUBMITTER: Cuylen-Haering S 

PROVIDER: S-EPMC7666080 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature