Transcriptional and Cellular Diversity of the Human Heart.

ABSTRACT:

Background

The human heart requires a complex ensemble of specialized cell types to perform its essential function. A greater knowledge of the intricate cellular milieu of the heart is critical to increase our understanding of cardiac homeostasis and pathology. As recent advances in low-input RNA sequencing have allowed definitions of cellular transcriptomes at single-cell resolution at scale, we have applied these approaches to assess the cellular and transcriptional diversity of the nonfailing human heart.

Methods

Microfluidic encapsulation and barcoding was used to perform single nuclear RNA sequencing with samples from 7 human donors, selected for their absence of overt cardiac disease. Individual nuclear transcriptomes were then clustered based on transcriptional profil

SUBMITTER: Tucker NR 

PROVIDER: S-EPMC7666104 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature