Visualisation of H₂O₂ penetration through skin indicates importance to develop pathway-specific epidermal sensing.

ABSTRACT: Elevated amounts of reactive oxygen species (ROS) including hydrogen peroxide (H₂O₂) are observed in the epidermis in different skin disorders. Thus, epidermal sensing of H₂O₂ should be useful to monitor the progression of skin pathologies. We have evaluated epidermal sensing of H₂O₂ in vitro, by visualising H₂O₂ permeation through the skin. Skin membranes were mounted in Franz cells, and a suspension of Prussian white microparticles was deposited on the stratum corneum face of the skin. Upon H₂O₂ permeation, Prussian white was oxidised to Prussian blue, resulting in a pattern of blue dots. Comparison of skin surface images with the dot patterns revealed that about 74% of the blue

SUBMITTER: Jankovskaja S 

PROVIDER: S-EPMC7666278 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature