Comparison of loop-mediated isothermal amplification and conventional PCR tests for diagnosis of common Brucella species.

ABSTRACT:

Objective

Rapid, reliable, and affordable detection of Brucella species via the molecular methods remains a challenge. In recent years, loop-mediated isothermal amplification (LAMP) is a functional nucleic acid amplification technique offering a substitute to polymerase chain reaction (PCR). So, we compared the LAMP assay with the conventional PCR for the identification of common Brucella species in Iran. In this study, LAMP assay was comprehensively evaluated against the common PCR method. A group of specific LAMP primers were used to amplify a highly specific fragment from the sequence of the Brucella abortus, bcsp31 gene. Sensitivity and specificity values of tests were done with a set of 78 (50 Brucella and 28 non-Brucella) strains.

Results

A dilution series of B. abortu

SUBMITTER: Moeini-Zanjani A 

PROVIDER: S-EPMC7666441 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature