Improving decolorization of dyes by laccase from Bacillus licheniformis by random and site-directed mutagenesis.

ABSTRACT:

Background

Dye wastewater increases cancer risk in humans. For the treatment of dyestuffs, biodegradation has the advantages of economy, high efficiency, and environmental protection compared with traditional physical and chemical methods. Laccase is the best candidate for dye degradation because of its multiple substrates and pollution-free products.

Methods

Here, we modified the laccase gene of Bacillus licheniformis by error-prone PCR and site-directed mutagenesis and expressed in E. coli. The protein was purified by His-tagged protein purification kit. We tested the enzymatic properties of wild type and mutant laccase by single factor test, and further evaluated the decolorization ability of laccase to acid violet, alphazurine A, and methyl orange by spectr

SUBMITTER: Bu T 

PROVIDER: S-EPMC7666548 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature