Genetic background impacts the timing of synaptonemal complex breakdown in Drosophila melanogaster.

ABSTRACT: Experiments performed in different genetic backgrounds occasionally exhibit failure in experimental reproducibility. This is a serious issue in Drosophila where there are no standard control stocks. Here, we illustrate the importance of controlling genetic background by showing that the timing of a major meiotic event, the breakdown of the synaptonemal complex (SC), varies in different genetic backgrounds. We assessed SC breakdown in three different control stocks and found that in one control stock, y w; sv^spa-pol, the SC broke down earlier than in Oregon-R and w¹¹¹⁸ stocks. We further examined SC breakdown in these three control backgrounds with flies heterozygous for a null mutation in c(3)G, which encodes a key structural component of the SC. Flies heterozygous fo

SUBMITTER: Wesley ER 

PROVIDER: S-EPMC7666587 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature