Exploring biomarkers and therapeutic targets for pressure overload induced heart failure based on microarray data.

ABSTRACT:

Background

Heart failure (HF) is an end stage heart condition with poor prognosis which brings about tremendous social medical cost. Along decades, mechanism and treatments of HF have been under restless research.

Methods

In the present study, we first established pressure overload induced HF model using transaortic arch constriction (TAC) method in mice. The global expression profiles of long noncoding RNA (lncRNA), microRNA (miRNA) and messenger RNA (mRNA) were obtained by microarray probes, which were further confirmed by quantitative PCR (qPCR). Bioinformatics analysis was performed using multiple methods including volcano plotting, heatmapping and hierarchical clustering, Gene Ontology (GO) and pathway enrichment analysis, and competing endogenous RNA (ceRNA) regulatory

SUBMITTER: He Y 

PROVIDER: S-EPMC7666961 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature