Subcellular Localization and Assembly Process of the Nisin Biosynthesis Machinery in Lactococcus lactis.

ABSTRACT: Nisin, a class I lantibiotic, is synthesized as a precursor peptide by a putative membrane-associated lanthionine synthetase complex consisting of the dehydratase NisB, the cyclase NisC, and the ABC transporter NisT. Here, we characterize the subcellular localization and the assembly process of the nisin biosynthesis machinery in Lactococcus lactis by mutational analyses and fluorescence microscopy. Precursor nisin, NisB, and NisC were found to be mainly localized at the cell poles, with a preference for the old poles. They were found to be colocalized at the same spots in these old pole regions, functioning as a nisin modification complex. In contrast, the transporter NisT was found to be distributed uniformly and circumferentially in the membrane. When nisin secretion was blocked

SUBMITTER: Chen J 

PROVIDER: S-EPMC7667030 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature