Different Routes of Protein Folding Contribute to Improved Protein Production in Saccharomyces cerevisiae.

ABSTRACT: Protein folding is often considered the flux controlling process in protein synthesis and secretion. Here, two previously isolated Saccharomyces cerevisiae strains with increased α-amylase productivity were analyzed in chemostat cultures at different dilution rates using multi-omics data. Based on the analysis, we identified different routes of the protein folding pathway to improve protein production. In the first strain, the increased abundance of proteins working on the folding process, coordinated with upregulated glycogen metabolism and trehalose metabolism, helped increase α-amylase productivity 1.95-fold compared to the level in the original strain in chemostat culture at a dilution rate of 0.2/h. The second strain further strengthened the folding precision to improve protein

SUBMITTER: Qi Q 

PROVIDER: S-EPMC7667031 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature