MyoSight-semi-automated image analysis of skeletal muscle cross sections.

ABSTRACT:

Background

Manual analysis of cross-sectional area, fiber-type distribution, and total and centralized nuclei in skeletal muscle cross sections is tedious and time consuming, necessitating an accurate, automated method of analysis. While several excellent programs are available, our analyses of skeletal muscle disease models suggest the need for additional features and flexibility to adequately describe disease pathology. We introduce a new semi-automated analysis program, MyoSight, which is designed to facilitate image analysis of skeletal muscle cross sections and provide additional flexibility in the analyses.

Results

We describe staining and imaging methods that generate high-quality images of immunofluorescent-labelled cross sections from mouse skeletal muscle. Using th

SUBMITTER: Babcock LW 

PROVIDER: S-EPMC7667765 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature