Moderate SMFs attenuate bone loss in mice by promoting directional osteogenic differentiation of BMSCs.

ABSTRACT:

Background

Osteoporosis is a common metabolic bone disease without effective treatment. Bone marrow-derived mesenchymal stem cells (BMSCs) have the potential to differentiate into multiple cell types. Increased adipogenic differentiation or reduced osteogenic differentiation of BMSCs might lead to osteoporosis. Whether static magnetic fields (SMFs) might influence the adipo-osteogenic differentiation balance of BMSCs remains unknown.

Methods

The effects of SMFs on lineage differentiation of BMSCs and development of osteoporosis were determined by various biochemical (RT-PCR and Western blot), morphological (staining and optical microscopy), and micro-CT assays. Bioinformatics analysis was also used to explore the signaling pathways.

Results

In this study, we found tha

SUBMITTER: Chen G 

PROVIDER: S-EPMC7667787 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature