Sperm miRNAs- potential mediators of bull age and early embryo development.

ABSTRACT:

Background

Sperm miRNAs were reported to regulate spermatogenesis and early embryonic development in some mammals including bovine. The dairy cattle breeding industry now tends to collect semen from younger bulls under high selection pressure at a time when semen quality may be suboptimal compared to adult bulls. Whether the patterns of spermatic miRNAs are affected by paternal age and/or impact early embryogenesis is not clear. Hence, we generated small non-coding RNA libraries of sperm collected from same bulls at 10, 12, and 16 months of age, using 16 months as control for differential expression and functional analysis.

Results

We firstly excluded all miRNAs present in measurable quantity in oocytes according to the literature. Of the remaining miRNAs, ten sperm-borne mi

SUBMITTER: Wu C 

PROVIDER: S-EPMC7667858 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature