Cold-induced chromatin compaction and nuclear retention of clock mRNAs resets the circadian rhythm.

ABSTRACT: Cooling patients to sub-physiological temperatures is an integral part of modern medicine. We show that cold exposure induces temperature-specific changes to the higher-order chromatin and gene expression profiles of human cells. These changes are particularly dramatic at 18°C, a temperature synonymous with that experienced by patients undergoing controlled deep hypothermia during surgery. Cells exposed to 18°C exhibit largely nuclear-restricted transcriptome changes. These include the nuclear accumulation of mRNAs encoding components of the negative limbs of the core circadian clock, most notably REV-ERBα. This response is accompanied by compaction of higher-order chromatin and hindrance of mRNPs from engaging nuclear pores. Rewarming reverses chromatin compaction and releases the transcr

SUBMITTER: Fischl H 

PROVIDER: S-EPMC7667876 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature