Single-Cell Transcriptional Archetypes of Airway Inflammation in Cystic Fibrosis.

ABSTRACT: Rationale: Cystic fibrosis (CF) is a life-shortening, multisystem hereditary disease caused by abnormal chloride transport. CF lung disease is driven by innate immune dysfunction and exaggerated inflammatory responses that contribute to tissue injury. To define the transcriptional profile of this airway immune dysfunction, we performed the first single-cell transcriptome characterization of CF sputum.Objectives: To define the transcriptional profile of sputum cells and its implication in the pathogenesis of immune function and the development of CF lung disease.Methods: We performed single-cell RNA sequencing of sputum cells from nine subjects with CF and five healthy control subjects. We applied novel computational approaches to define expression-based cell function a

SUBMITTER: Schupp JC 

PROVIDER: S-EPMC7667912 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature