Human Wharton's jelly mesenchymal stem cells protect neural cells from oxidative stress through paracrine mechanisms.

ABSTRACT:

Aim

Mesenchymal stem cells (MSCs) have neuroprotective and immunomodulatory properties, which are partly mediated by extracellular vesicles (EVs) secretion. We aimed to evaluate the effects of human Wharton's jelly-derived MSCs (WJ-MSCs) and their EVs on rat hippocampal cultures subjected to hydrogen peroxide (H₂O₂).

Materials & methods

Hippocampal dissociated cultures were either co-cultured with WJ-MSCs or treated with their EVs prior to H₂O₂ exposure and reactive oxygen species levels and cell viability were evaluated.

Results

Coculture with WJ-MSCs or pre-incubation with EVs prior to the insult reduced reactive oxygen species after H₂O₂ exposure. Cell viability was improved only when coculture was m

SUBMITTER: Puig-Pijuan T 

PROVIDER: S-EPMC7668126 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature