Stress-dependent conformational changes of artemin: Effects of heat and oxidant.

ABSTRACT: Artemin is an abundant thermostable protein in Artemia embryos and it is considered as a highly efficient molecular chaperone against extreme environmental stress conditions. The conformational dynamics of artemin have been suggested to play a critical role in its biological functions. In this study, we have investigated the conformational and functional changes of artemin under heat and oxidative stresses to identify the relationship between its structure and function. The tertiary and quaternary structures of artemin were evaluated by fluorescence measurements, protein cross-linking analysis, and dynamic light scattering. Based on the structural analysis, artemin showed irreversible substantial conformational lability in responses to heat and oxidant, which was mainly mediated through th

SUBMITTER: Takalloo Z 

PROVIDER: S-EPMC7668597 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature