Ion transport mechanisms for smoke inhalation-injured airway epithelial barrier.

ABSTRACT: Smoke inhalation injury is the leading cause of death in firefighters and victims. Inhaled hot air and toxic smoke are the predominant hazards to the respiratory epithelium. We aimed to analyze the effects of thermal stress and smoke aldehyde on the permeability of the airway epithelial barrier. Transepithelial resistance (R_TE) and short-circuit current (I_SC) of mouse tracheal epithelial monolayers were digitized by an Ussing chamber setup. Zonula occludens-1 tight junctions were visualized under confocal microscopy. A cell viability test and fluorescein isothiocyanate-dextran assay were performed. Thermal stress (40 °C) decreased R_TE in a two-phase manner. Meanwhile, thermal stress increased I_SC followed by its decline. Na⁺ depletion,

SUBMITTER: Chang J 

PROVIDER: S-EPMC7669642 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature