Deletion of JNK Enhances Senescence in Joint Tissues and Increases the Severity of Age-Related Osteoarthritis in Mice.

ABSTRACT:

Objective

To determine the role of JNK signaling in the development of osteoarthritis (OA) induced by joint injury or aging in mice.

Methods

In the joint injury model, 12-week-old wild-type control, JNK1^-/- , JNK2^-/- , and JNK1^fl/fl JNK2^-/- aggecan-Cre^{ERT 2} double-knockout mice were subjected to destabilization of the medial meniscus (DMM) (n = 15 mice per group) or sham surgery (n = 9-10 mice per group), and OA was evaluated 8 weeks later. In the aging experiment, wild-type control, JNK1^-/- , and JNK2^-/- mice (n = 15 per group) were evaluated at 18 months of age. Mouse knee joints were evaluated by scoring articular cartilage structure, toluidine blue staining, osteophytes, and synovial

SUBMITTER: Loeser RF 

PROVIDER: S-EPMC7669715 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature