Chromatin Potential Identified by Shared Single-Cell Profiling of RNA and Chromatin.

ABSTRACT: Cell differentiation and function are regulated across multiple layers of gene regulation, including modulation of gene expression by changes in chromatin accessibility. However, differentiation is an asynchronous process precluding a temporal understanding of regulatory events leading to cell fate commitment. Here we developed simultaneous high-throughput ATAC and RNA expression with sequencing (SHARE-seq), a highly scalable approach for measurement of chromatin accessibility and gene expression in the same single cell, applicable to different tissues. Using 34,774 joint profiles from mouse skin, we develop a computational strategy to identify cis-regulatory interactions and define domains of regulatory chromatin (DORCs) that significantly overlap with super-enhancers. During lineage comm

SUBMITTER: Ma S 

PROVIDER: S-EPMC7669735 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature