Histo-ELISA technique for quantification and localization of tissue components.

ABSTRACT: A novel Histo-ELISA technique is intended to facilitate quantification of target tissue proteins in a tissue section and involves the selection of target regions in the tissue section, application of streptavidin-conjugated HRP (horseradish peroxidase), coupled with peroxidase substrate-TMB (3,3',5,5'-tetramethylbenzidine), and staining dye evaluation with ELISA reader. The target protein content (weight per volume unit) was translated from optical densities by a reference standard curve, obtained via parallel staining of the targeted protein-coated slides. To validate the technique, we carried out quantifications of IgG extravasation in ischemic and nonischemic brain sections in a mouse stroke model. With those obtained data and the reference of immunohistochemistry scores assessed on the

SUBMITTER: Li Z 

PROVIDER: S-EPMC7669848 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature