Identification of Ginger (Zingiber officinale Roscoe) Reference Genes for Gene Expression Analysis.

ABSTRACT: Quantitative real-time PCR (qRT-PCR) is widely used in the detection of gene expression level. However, there is no suitable ginger reference gene for qPCR analysis. Therefore, it is the primary task to select and validate the appropriate ginger reference gene to normalize the expression of target genes. In this study, 14 candidate reference genes were selected and analyzed in different tissues (leaf, and rhizome), different development stages, different varieties, and abiotic stress (ABA and salt stress). Expression stability was calculated using geNorm and NormFinder, Bestkeeper, and RefFinder. For abiotic stress and total conditions, 28S and COX were identified as the most stable genes. In addition, RPII was the most stable in the different development stages and different varieties. TE

SUBMITTER: Lv Y 

PROVIDER: S-EPMC7670040 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature