Inflammatory Markers in Suction Blister Fluid: A Comparative Study Between Interstitial Fluid and Plasma.

ABSTRACT:

Background

Biomarker analysis allows for the detection and prediction of disease as well as health monitoring. The use of interstitial fluid (ISF) as a matrix for biomarkers has recently gained interest. This study aimed to compare levels of inflammatory markers in ISF from suction blister fluid (SBF) and plasma.

Methods

Plasma and SBF were collected from 18 healthy individuals. Samples were analyzed for 92 inflammation-related protein biomarkers by Proximity Extension Assay (PEA). Protein profiles in the two matrices were compared using traditional and multivariate statistics.

Results

Out of 92 targeted proteins, 70 were successfully quantified in both plasma and SBF. Overall, plasma and SBF displayed distinct protein profiles with up to 40-fold difference in abundan

SUBMITTER: Sjobom U 

PROVIDER: S-EPMC7670055 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature