ATXN7L3 positively regulates SMAD7 transcription in hepatocellular carcinoma with growth inhibitory function.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC) is a leading cause of cancer death worldwide, with unmet need for the pharmacological therapy. The functions of ATXN7L3 in HCC progression are not known.

Methods

RNA sequence, quantitative real-time PCR, and western blot were performed to detect gene expression. Chromatin immunoprecipitation was performed to detect possible mechanisms. Immunohistochemical stain was performed to examine the protein expression. Colony formation, cell growth curve and xenograft tumor experiments were performed to examine cell growth in vitro and in vivo.

Findings

ATXN7L3 functions as a coactivator for ERα-mediated transactivation in HCC cells, thereby contributing to enhanced SMAD7 transcription. ATXN7L3 is recruited to the promoter regions of SM

SUBMITTER: Sun N 

PROVIDER: S-EPMC7670205 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature