Isolation and identification of beneficial orchid mycorrhizal fungi in Bletilla striata (Thunb.) Rchb.f.(Orchidaceae).

ABSTRACT: Orchid mycorrhizal fungi (OMF) are essential for orchids growth. Bletilla striata (Thunb.) Rchb.f.(Orchidaceae) has high ornamental and medicinal value. Beneficial OMF isolation is crucial to improve the survival rate of B. striata tissue culture and transplanting. In this study, we isolated and identified the beneficial OMF in B. striata from the roots of sterilized wild B. striata seedlings by culturing in four different mediums. The germination states of B. striata seeds inoculated with diverse OMF were classified and calculated. Fresh and dry weight increments of B. striata seedlings inoculated with diverse OMF were recorded after 90 d of culturing on 1/2 MS medium. ITS sequences of beneficial fungi were amplified by PCR and taxonomically ident

SUBMITTER: Xi G 

PROVIDER: S-EPMC7671041 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature