CasCollect: targeted assembly of CRISPR-associated operons from high-throughput sequencing data.

ABSTRACT: CRISPR arrays and CRISPR-associated (Cas) proteins comprise a widespread adaptive immune system in bacteria and archaea. These systems function as a defense against exogenous parasitic mobile genetic elements that include bacteriophages, plasmids and foreign nucleic acids. With the continuous spread of antibiotic resistance, knowledge of pathogen susceptibility to bacteriophage therapy is becoming more critical. Additionally, gene-editing applications would benefit from the discovery of new cas genes with favorable properties. While next-generation sequencing has produced staggering quantities of data, transitioning from raw sequencing reads to the identification of CRISPR/Cas systems has remained challenging. This is especially true for metagenomic data, which has the highest poten

SUBMITTER: Podlevsky JD 

PROVIDER: S-EPMC7671303 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature