Identification and analysis of RNA structural disruptions induced by single nucleotide variants using Riprap and RiboSNitchDB.

ABSTRACT: RNA conformational alteration has significant impacts on cellular processes and phenotypic variations. An emerging genetic factor of RNA conformational alteration is a new class of single nucleotide variant (SNV) named riboSNitch. RiboSNitches have been demonstrated to be involved in many genetic diseases. However, identifying riboSNitches is notably difficult as the signals of RNA structural disruption are often subtle. Here, we introduce a novel computational framework-RIboSNitch Predictor based on Robust Analysis of Pairing probabilities (Riprap). Riprap identifies structurally disrupted regions around any given SNVs based on robust analysis of local structural configurations between wild-type and mutant RNA sequences. Compared to previous approaches, Riprap shows higher accuracy when a

SUBMITTER: Lin J 

PROVIDER: S-EPMC7671322 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature