Identifying microbial species by single-molecule DNA optical mapping and resampling statistics.

ABSTRACT: Single-molecule DNA mapping has the potential to serve as a powerful complement to high-throughput sequencing in metagenomic analysis. Offering longer read lengths and forgoing the need for complex library preparation and amplification, mapping stands to provide an unbiased view into the composition of complex viromes and/or microbiomes. To fully enable mapping-based metagenomics, sensitivity and specificity of DNA map analysis and identification need to be improved. Using detailed simulations and experimental data, we first demonstrate how fluorescence imaging of surface stretched, sequence specifically labeled DNA fragments can yield highly sensitive identification of targets. Second, a new analysis technique is introduced to increase specificity of the analysis, allowing even closely re

SUBMITTER: Bouwens A 

PROVIDER: S-EPMC7671359 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature