In silico analysis and expression of a new chimeric antigen as a vaccine candidate against cutaneous leishmaniasis.

ABSTRACT:

Objectives

Since leishmaniasis is one of the health problems in many countries, the development of preventive vaccines against it is a top priority. Peptide vaccines may be a new way to fight the Leishmania infection. In this study, a silicon method was used to predict and analyze B and T cells to produce a vaccine against cutaneous leishmaniasis.

Materials and methods

Immunodominant epitope of Leishmania were selected from four TSA, LPG3, GP63, and Lmsti1 antigens and linked together using a flexible linker (SAPGTP). The antigenic and allergenic features, 2D and 3D structures, and physicochemical features of a chimeric protein were predicted. Finally, through bioinformatics methods, the mRNA structure was predicted and was produced chemically and cloned into the pLEX

SUBMITTER: Motamedpour L 

PROVIDER: S-EPMC7671421 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature