A Rigorous Quantitative Approach to Analyzing Phagocytosis Assays.

ABSTRACT: Studying monocytic cells in isolated systems in vitro contributes significantly to the understanding of innate immune physiology. Functional assays produce read outs which can be used to measure responses to selected stimuli, such as pathogen exposure, antigen loading, and cytokine stimulation. Integration of these results with high quality in vivo models allows for the development of therapeutics which target these cell populations. Current methodologies to quantify phagocytic function of monocytic cells in vitro either measure phagocytic activity of individual cells (average number of beads or particles/cell), or a population outcome (% cells that contain phagocytosed material). Here we address technical challenges and shortcomings of these methods and present a prot

SUBMITTER: Caponegro MD 

PROVIDER: S-EPMC7671587 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature