Survival Association and Cell Cycle Effects of B7H3 in Neuroblastoma.

ABSTRACT:

Objective

The function of B7H3, a member of the B7 family of proteins, in neuroblastoma (NB) remains poorly characterized. Here we examine the expression pattern of B7H3 in clinical NB specimens and characterize the phenotype of B7H3 knock-down in NB cell line.

Methods

Immunohistochemical (IHC) staining was carried out to assess the expression of B7H3 in clinical NB specimens. Survival association was analyzed using five Gene Expression Omnibus (GEO) datasets (GSE85047, GSE45480, GSE62564, GSE16476, GSE49710). Clonogenic survival and flow cytometry were performed after B7H3 knockdown to assess the cellular proliferation and cell survival in vitro. Impact of B7H3 silencing on NB growth was examined in vivo using the SH-SY5Y xenograft model.

Results

On IHC staining, B7H

SUBMITTER: Zhang H 

PROVIDER: S-EPMC7671786 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature