Partially Differentiated Neuroretinal Cells Promote Maturation of the Retinal Pigment Epithelium.

ABSTRACT:

Purpose

Many studies have demonstrated the ability of the retinal pigment epithelium (RPE) to foster the maturation of the developing retina. Few studies have examined the reciprocal effects of developing retina on the RPE.

Methods

RPE isolated from human fetal RPE or differentiated from human stem cells was cultured on Transwell filter inserts. Retinal progenitor cells (RPCs) were differentiated from human stem cells and cultured on a planar scaffold composed of gelatin, chondroitin sulfate, hyaluronic acid, and laminin-521. Cultures were analyzed by quantitative RT-PCR, immunofluorescence, immunoblotting, and transepithelial electrical resistance (TER).

Results

RPCs initially differentiated into several retina-like cell types that segregated from one another and for

SUBMITTER: Singh D 

PROVIDER: S-EPMC7671856 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature