Identification of NLRP3 Inflammation-Related Gene Promoter Hypomethylation in Diabetic Retinopathy.

ABSTRACT:

Purpose

To identify and validate key genes that could provide a new perspective for genetic marker screening of diabetic retinopathy (DR).

Methods

The gene expression and DNA methylation profiles were obtained from the Gene Expression Omnibus. Differential expression analysis was conducted using the limma package, and then the functions of the differentially expressed genes (DEGs) were analyzed using the DAVID database, followed by protein-protein interaction (PPI) networks using Cytoscape software. We employed the Sequenom MassARRAY system to detect the promoter methylation levels of the candidate genes in peripheral blood mononuclear cells from 32 healthy individuals and 94 patients with type 2 diabetes mellitus (T2D; 64 with DR and 30 without DR) and in fibrovascular memb

SUBMITTER: Chen H 

PROVIDER: S-EPMC7671867 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature