Sigma-1 receptor activity in primary sensory neurons is a critical driver of neuropathic pain.

ABSTRACT: The Sigma-1 receptor (σ₁R) is highly expressed in the primary sensory neurons (PSNs) that are the critical site of initiation and maintenance of pain following peripheral nerve injury. By immunoblot and immunohistochemistry, we observed increased expression of both σ₁R and σ₁R-binding immunoglobulin protein (BiP) in the lumbar (L) dorsal root ganglia (DRG) ipsilateral to painful neuropathy induced by spared nerve injury (SNI). To evaluate the therapeutic potential of PSN-targeted σ₁R inhibition at a selected segmental level, we designed a recombinant adeno-associated viral (AAV) vector expressing a small hairpin RNA (shRNA) against rat σ₁R. Injection of this vector into the L4/L5 DRGs induced downregulation of σ₁R in DRG neu

SUBMITTER: Shin SM 

PROVIDER: S-EPMC7671947 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature