In silico design and validation of a highly degenerate primer pair: a systematic approach.

ABSTRACT:

Background

The techniques of amplifying genetic materials have enabled the extensive study of several biological activities outside the biological milieu of living systems. More recently, this approach has been extended to amplify population of genes, from evolutionarily related gene family for detection and evaluation of microbial consortial with several unique potentialities (e.g., enzymatic degradability). Conceivably, primer mixtures containing substitutions of different bases at specific sites (degenerate primers) have enabled the amplification of these genes in PCR reaction. However, the degenerate primer design problem (DPD) is a constraint to designing this kind of primer. To date, different algorithms now exist to solve various versions of DPD problem, many of which, only

SUBMITTER: Chukwuemeka PO 

PROVIDER: S-EPMC7671957 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature